Date of Graduation
2000
Document Type
Thesis
Degree Type
MS
Committee Chair
Yon Rojanasakul
Abstract
The overall goal of this project was to investigate the feasibility of a new strategy to inhibit the NF-κB signal transduction pathway. Peptide P1 that resembled IκBα , the intracellular inhibitor’s kinase binding domain was designed to competitively inhibit IκBα’s degradation. Preliminary results in stably transfected cells indicated that P1 produced a two-fold inhibition in NF-κB dependent luciferase activity in Lipopolysaccharide (LPS) stimulated RAW cell line, but not in BEAS-2B cell line. A transient transfection protocol was optimized in the BEAS-2B cell line and the effect of P1 was compared to SN50 a known inhibitor of NF-κB pathway. Our results showed that P1 produced a more potent inhibition in these studies. TNF-α ELISA results also indicated that P1 inhibited NF-κB dependent TNF-α production. Western Blot results indicated that LPS stimulated degradation of IκBα was also inhibited by P1 but not by P2, a control peptide indicating that our peptide was both potent and specific.
Recommended Citation
Swaroop, Navin V., "Nuclear factor-κB signal transduction: Development of a novel regulatory strategy." (2000). Graduate Theses, Dissertations, and Problem Reports. 10532.
https://researchrepository.wvu.edu/etd/10532